Coding

Part:BBa_K4170001:Design

Designed by: Alexandros Giannopoulos Dimitriou   Group: iGEM22_Thessaloniki_Meta   (2022-09-27)


bdSUMO solubility tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 289
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SUMO coding sequence can be fused to the N-terminus of any desired recombinant protein.


Source

This part was amplified utilizing suitable primers from pGJK_His-SUMO-LbuCas13a plasmid (addgene#172488)

References